Toxoplasma Gondii Antibody (TOXO Ab) Test Kit

Toxoplasma Gondii Antibody (TOXO Ab) Test Kit can quickly and qualitatively detect toxoplasma antibodies in dog or cat serum for screening and auxiliary diagnosis of toxoplasma infection.

【testing principle】

Toxoplasmosis in dogs and cats is a zoonotic parasitic disease caused by toxoplasmosis. The main manifestations are fever, anorexia, depression, vomiting, diarrhea, feces mixed with blood, liquid, cough, eyes and nose secretions, dyspnea, visual mucosa pale; Some have iritis and even blindness. Toxoplasma gondii reproduces sexually and gametes in the cat's gut, develops into an egg sac, and is excreted in the feces. Under suitable conditions, it develops into infectious sporogenous oocysts after sporulation. After being swallowed by healthy dogs and cats, oocysts escape in the intestine, enter the tissues of the body with the blood circulation, invade cells and rapidly divide and proliferate, and open into intracellular pseudocysts, causing clinical symptoms.

This kit uses double antibody sandwich immunochromatography. If the sample contains enough Toxoplasma antibodies, the antibodies will bind to the Toxoplasma antigen coated with colloidal gold on the gold label pad, forming an antigen-antibody complex. When this complex migrates upward to the detection area (T-line) with the capillary effect, it binds to another antigen to form an "antigen-antibody-antigen" complex and gradually agglutinates into a visible detection line (T-line), and excess colloidal gold antigen continues to migrate to the quality control area (C-line) to be captured by monoclonal antibody and form a visible C-line. The test results are displayed on the C and T lines. The red band displayed by the quality control line (C line) is the standard to determine whether the chromatographic process is normal, and also serves as the internal control standard of the product.

【 Package Specifications and components 】

Note: swabs are complimentary separately according to package specifications.

【Storage and expiration date】

The kit is stored at 2-30℃. Do not freeze. Valid for 24 months; After the kit is opened, the reagent should be used as soon as possible.

【Sample requirement】

1.  Sample: Dog (cat) serum.

2. Samples should be tested on the same day; Samples that cannot be tested on the same day should be stored at 2-8 ° C, and those that exceed 24 hours should be stored at -20 ° C.

【Inspection method】

1.  Before use, restore the kit to room temperature (15-30℃).

2.  Remove the reagent card from the aluminum foil bag and place it on a clean platform.

3.  Unscrew the top tube cap on the diluent tube cap containing the sample, invert the diluent tube, squeeze the tube wall, and add 3-5 drops of specimen mixture into the sample hole (S hole) of the reagent card.

4.  The results can be read in 10-15 minutes.  The result is invalid after 15 minutes.

【 Result interpretation 】

Positive: Both the quality control line (C line) and the test line (T line) appear

Negative: Only the quality control line (C line) is available

Invalid: Quality control line does not appear, take a new device to retest

【Precautions】  

1.   This product is only used for qualitative testing and does not indicate the virus level in the sample.

2.   Test results of this product are for reference only and should not be used as the sole basis for diagnosis and treatment, but should be made by a physician after evaluating all clinical and laboratory evidence.

3.   A negative result may occur if the viral antigen present in the sample is below the detection limit of the assay, or if the antigen detected at the disease stage at which the sample was collected is not present.

4.   The operation should be carried out in strict accordance with the instructions.   Do not use expired or damaged products.

5.   The test card should be used within 1 hour after opening;   If the ambient temperature is higher than 30 ° C or more humid, it should be used immediately.

6.   If the T line has just begun to show color, and then the line color gradually fades or even disappears, in this case, the sample should be diluted several times and tested until the T line color is stable.

7.   This product is a disposable product.   Do not reuse it.